Characterization of the plastid terminal oxidase gene in carrot-involvement in carotenoids accumulation during storage root development
Keywords
- Daucus carota
- amino acids
- biosynthesis
- carotenoids
- carrots
- chloroplasts
- cultivars
- exons
- gene expression regulation
- genes
- iron
- sequence analysis
Notes
p. 85-92.
Publication Dbxref
AGL:5867023
Title
Characterization of the plastid terminal oxidase gene in carrot-involvement in carotenoids accumulation during storage root development
Publication Type
Journal Article
Series Name
Acta horticulturae
Publication Year
2017
Issue
1153
Page Numbers
85-92
Publication Date
2017
Citation
Campos MD, Cardoso HG, Nogales A, Campos C, Arnholdt-Schmitt B. Characterization of the plastid terminal oxidase gene in carrot-involvement in carotenoids accumulation during storage root development. Acta horticulturae. 2017; (1153)85-92.
Language Abbr
eng
Publication Model
[electronic resource].
Abstract
The plastid terminal oxidase (PTOX) gene is known to be implicated in the carotenoid biosynthesis pathway. In the present work we characterize the Daucus carota L. PTOX gene (DcPTOX) and describe the relation between DcPTOX transcript level and carotenoids accumulation during storage root development of white, yellow, orange, red, and purple carrot cultivars. Transcript analysis of five genes (PSY2, PDS, ZDS, LCYB, LCYE) known as being directly involved in carotenoid biosynthesis pathways was also performed. PTOX sequence analysis reveals the presence of typical structural features of PTOXs from higher plants: conserved iron binding residues, conserved exon 8 amino acids and 6 conserved cysteins at the C-terminal region, and the presence of a chloroplast transit peptide at the N-terminus. Expression analysis revealed the presence of DcPTOX transcripts in all cultivars, and an increase of transcripts during the time course of the experiment, with differential expression among cultivars in early stages of root growth. Our results demonstrated that DcPTOX has similar profile to that of other carotenoid biosynthetic genes with high correlation to all.
Relationship
The Publication Campos MD, Cardoso HG, Nogales A, Campos C, Arnholdt-Schmitt B. Characterization of the plastid terminal oxidase gene in carrot-involvement in carotenoids accumulation during storage root development. Acta horticulturae. 2017; (1153)85-92. is a part of Publication Briard M. Ed.. International Symposium on Carrot and Other Apiaceae. Acta Horticulturae. 2017 Mar; 1153.
Database Reference Annotations
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