NCBI accession DQ898156; Daucus carota chloroplast, complete genome.

Core Data

plastid_assembly

Name

Type

genome assembly

Description

Assembly deposited as NCBI GenBank accession DQ898156

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DNA was sheared by nebulization, size fractionated to 4–6 kb, linker ligated and cloned into pHOS2, a TIGR medium copy vector. A total of 1231 high quality reads with an average length of 808 bases was generated during the random (1126 reads) and closure (105 reads) phases of sequencing. Sequences were assembled using TIGR assembler and scaffolded using Bambus. Sequence finishing included directed PCR to span gaps and directed primer walking of clones to cover the entire genome and to complete regions of low depth of coverage.

Program, Pipeline, Workflow or Method Name

TIGR assembler + Bambus

Program Version

DQ898156

Data Source Name

NCBI accession DQ898156

Data Source Version

DQ898156.1

Database Reference Annotations

Publication

Ruhlman T, Lee SB, Jansen RK, Hostetler JB, Tallon LJ, Town CD, Daniell H. Complete plastid genome sequence of Daucus carota: implications for biotechnology and phylogeny of angiosperms.. BMC genomics. 2006 Aug 31; 7:222.

Organism

Daucus carota