1993 Celery RFLP

Linkage relationships are reported for 34 markers in celery (Apium graveolens L. var 'dulce') including 21 RFLP, 11 isozyme, and 2 morphological traits. The mapping was carried out in a cross between celery and an annual accession from Thailand, A143, and based on F₂ segregation of 136 plants. A total of 318 centiMorgans (cM) are covered by the markers distributed in 8 linkage groups. Probes for the identification of RFLPs were isolated from a celery cDNA library and were also obtained from heterologous sources. EcoRV, EcoRI, and HindIII were the most useful restriction enzymes in uncovering polymorphism. In our cross, 18% of the cDNA probes were found to be polymorphic for at least one of the enzymes used. Six of the markers showed significant deviations from expected F₂ ratios.