(1)the proton nuclear magnetic resonance-pattern recognition research of rhodiola crendata from different districts (2)the proton nuclear magnetic resonance-pattern recognition research of angelica dahurica var. formosana from different districts

Translation from original language as provided by author This dissertation was composed of two parts. Chapter one of the first part describes The Proton Nuclear Magnetic Resonance-Pattern Recognition Research of Rhodiola crendata from Different Districts. The drug actions of medicinal plants were based on its effective constituents, most of which were the secondary metabolites of plants. Because some environmental factors such as altitude, climate and soil will affect the metabolites of plants, there will be some differences between medicinal plants from different growing areas. The plant Rhodiola rosea belongs to the genus Rhodiola L. of Crassulaceae. The whole plant may be medicine. Rhodiola crendata is recored as the standard species of Rhodiola rosea in Tibetan medicine standards, which was promulgated by China's Ministry of Health. In order to study the influence of the environmental factors on the chemical compositions of Rhodiola crendata in a comprehensive perspective, principal component analysis (PCA) were employed to perform the analysis of 1H NMR of Rhodiola crendata samples from 12 different areas. Samples from different growing areas could be distinguished by this method and the results display the difference of the samples. This study determined the categories of chemical components which led to the difference between samples. According the results of analysis, we have compared the content of carbohydrate and flavone in these samples. This study has laid the foundation for further researches, in which we will combine the results of analysis with many environmental factors to identify the most influential factors to Rhodiola crendata and could guide the selection and planting of this medicinal plant. Chapter tow of the first part was the study on quality evaluation of Angelica dahurica var. using 1H NMR spectra with pattern recognition research. Traditional Chinese medicine has been widely used in clinical practice. But because of the influence of species, environment, culture conditions, processing methods and other factors, the quality of traditional Chinese drug in the market is mixed, the drug actions can't be guaranteed. So the drug quality controlling is a necessary work. Currently, the main quality control methods for traditional Chinese drug are thin-layer chromatography (TLC), high performance liquid chromatography (HPLC), gas chromatography (GC) and so on. These methods can only detect one or several components at one time, the results are not the comprehensive. Proton nuclear magnetic resonance (1H NMR) combined with pattern recognition is a research methods based on all chemical compositions of traditional Chinese drug. The traditional Chinese drug Angelicae dahuricae radix is the dry root of the plant Angelica dahurica or Angelica dahurica var. formosana. The latter is a cultivated variety of the former and it is the mainstream variety. The quality of the crude drug varies from areas and the best Angelicae dahuricae radix was produced in Suining, Sichuan province. In order to avoid vermin, control mould and whitening the crude drug, people usually fumigate it with burning sulfur. This may lead some changes in chemical compositions of the crud drug. The rationality of this treatment should be studied. In order to establish a quality evaluation method based on all chemical compositions of Angelicae dahuricae radix and study the influence of the sulfur fumigation on the chemical compositions, PCA were employed to perform the analysis of 1H NMR of Angelica dahurica var. formosana samples from different areas. By this method the fumigated and non-fumigated samples could be distinguished, and the non-fumigated samples from different areas could be distinguished too. The results indicate that both sulfur fumigation and area factors could lead the chemical compositions of crude drug change, and the influence of sulfur fumigation is greater. This study was of great significance in rationality evaluation of the processing method, it can also help to improve the quality control mode and cultivation methods. The second part of dissertation was a review about metabonomics in plant research. This review introduced the analytical instruments and pattern recognition methods in metabonomics research. It also enumerated some studies in plant metabonomics research recent