Development of genetic markers in celery based on restriction fragment length polymorphisms.
- Apium graveolens
- Apium
- Apium graveolens var. rapaceum
- restriction fragment length polymorphism
- loci
- isozymes
- genetic markers
- linkage groups
- anthocyanins
- linkage (genetics)
- inheritance (genetics)
- chromosome mapping
- segregation
- apium chilense
- apium panul
- apium graveolens var. secaliunum
Linkage relationships are reported for 34 markers in celery (Apium graveolens L. var 'dulce') including 21 RFLP, 11 isozyme, and 2 morphological traits. The mapping was carried out in a cross between celery and an annual accession from Thailand, A143, and based on F2 segregation of 136 plants. A total of 318 centiMorgans (cM) are covered by the markers distributed in 8 linkage groups. Probes for the identification of RFLPs were isolated from a celery cDNA library and were also obtained from heterologous sources. EcoRV, EcoRI, and HindIII were the most useful restriction enzymes in uncovering polymorphism. In our cross, 18% of the cDNA probes were found to be polymorphic for at least one of the enzymes used. Six of the markers showed significant deviations from expected F2 ratios.
- MK019127 Adh-2
- MK019128 Pgm-3
- MK019129 Pgi-3
- MK019130 Mdh-3
- MK019132 Sdh-1
- MK019133 Adh-1
- MK019134 Mdh-1
- MK019135 Got-1
- MK019136 Mdh-2
- MK019137 C30
- MK019138 5S
- MK019140 D1
- MK019141 D26
- MK019142 G28
- MK019143 F38
- MK019144 B5
- MK019145 F19
- MK019146 C22
- MK019147 PAL
- MK019148 C27
- MK019149 B2
- MK019150 F48
- MK019151 B22
- MK019152 Cab-1
- MK019153 B37
- MK019154 C37
- MK019155 G43
- MK019156 F34
- MK019157 D15
- MK019158 Tpi-1
- MK019159 B33
- Celery annual habit
- Celery purple petiole